The small GTPase CDC42 regulates actin dynamics during porcine oocyte maturation
نویسندگان
چکیده
The mammalian oocyte undergoes an asymmetric division during meiotic maturation, producing a small polar body and a haploid gamete. This process involves the dynamics of actin filaments, and the guanosine triphosphatase (GTPase) protein superfamily is a major regulator of actin assembly. In the present study, the small GTPase CDC42 was shown to participate in the meiotic maturation of porcine oocytes. Immunofluorescent staining showed that CDC42 was mainly localized at the periphery of the oocytes, and accumulated with microtubules. Deactivation of CDC42 protein activity with the effective inhibitor ML141 caused a decrease in actin distribution in the cortex, which resulted in a failure of polar body extrusion. Moreover, western blot analysis revealed that besides the Cdc42-N-WASP pathway previously reported in mouse oocytes, the expression of ROCK and p-cofilin, two molecules involved in actin dynamics, was also decreased after CDC42 inhibition during porcine oocyte maturation. Thus, our study demonstrates that CDC42 is an indispensable protein during porcine oocyte meiosis, and CDC42 may interact with N-WASP, ROCK, and cofilin in the assembly of actin filaments during porcine oocyte maturation.
منابع مشابه
Advance Publication by J-STAGE Journal of Reproduction and Development
23 The mammalian oocyte undergoes an asymmetric division during meiotic maturation, 24 producing a small polar body and a haploid gamete. This process involves the dynamics of 25 actin filaments, and the guanosine triphosphatase (GTPase) protein superfamily is a major 26 2 regulator of actin assembly. In the present study, the small GTPase CDC42 was shown to 1 participate in the meiotic maturat...
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